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. 2012 Oct 1;122(11):3919–3930. doi: 10.1172/JCI63888

Figure 4. An MFBD promoted autophagy in cardiomyocytes.

Figure 4

(A) Myocardial mRNA levels of the autophagy markers Map1lc3b (LC3B) and Becn1 (Beclin 1) were upregulated by the MFBD but not by the LBD. (B) Immunohistochemical and (C) immunofluorescent staining revealed increased numbers of LC3B puncta (indicated by arrows in B and enclosed in circles in C) in the MFBD but not the LBD after 18 weeks of high-fat feeding. This effect was attenuated by myriocin treatment. Photographs were taken in a cross-sectional plane with a ×10 objective (B) and in a transverse plane using a ×60 objective (C). Results are presented as mean ± SEM. *P < 0.05 vs. control.