Figure 2. Increased hepatic TAG content in chow-fed PNPLA3^I148M transgenic mice.

(A) Liver sections from 12-week-old chow-fed wild-type (+/+) mice and PNPLA3^WT and PNPLA3^I148M transgenic male mice (n = 4/group) were stained with Oil Red O and viewed using a Leica microscope (DM2000) (original magnification, ×63). Hepatic lipid levels were measured in the same mice after a 4-hour fast. *P < 0.05. (B) Sections from adipose tissue of 12-week-old chow-fed wild-type mice and A-PNPLA3^WT and A-PNPLA3^I148M mice (n = 4/group) were stained with hematoxylin and eosin (top row). Images of BAT (original magnification, ×40) and WAT (original magnification, ×20) were taken. Hepatic lipid levels were measured after a 4-hour fast. Values are mean ± SEM (n = 4/group). Levels were compared among lines using Student’s t tests. The experiment was performed 3 times (n = 4–5 mice/group), and results were similar.