Figure 7. Th9 cells regulate DC function in a Ccr6-dependent manner.

(A) C57BL/6 mice (n = 3/group) were i.v. challenged with 1 × 10⁵ B16-OVA-GFP tumor cells and, on the same day, were i.v. transferred with 3 × 10⁶ Th1 or Th9 cells. LLNs were tested using FACS 3 days later for DC populations. Histograms show the percentage of GFP⁺ DCs gated on CD11c⁺CD8α⁺ DCs and CD11c⁺CD11b⁺ DCs. (B) Number of total and GFP⁺ cells for each DC population was calculated from A. Representative results from one of two performed experiments are shown. (C) PBS or 3 × 10⁶ Th9 cells were transferred to mice (wild-type and Ccr6^–/– mice; n =4/group) bearing 5-day established pulmonary B16-OVA melanoma. Shown are the lung weights on day 19 after challenge. (D) Representative images of the lungs of mice receiving different treatments. (E) LLN cells from C were analyzed for DC populations using FACS. The numbers of total CD8α⁺ DCs and CD11b⁺ DCs from mice (n = 4/group) are shown. Unless otherwise indicated, P values are shown as indicated.