Fig. 3.

The effect of eupatilin, SB202190, SP600125 or NAC on the H₂O₂-induced 5-LOX expression and LTB₄ production. Serum-starved EECs were preincubated in the presence of eupatilin (150 µM, 12 hours), SB202190 (30 µM, 1 hours), SP600125 (30 µM, 1 hours), or NAC (5 mM, 1 hours). The cells were then stimulated with H₂O₂ (300 µM, 24 hours). (A) The change level of 5-LOX expression was estimated by Western blot analysis. (B) The production level of LTB₄ was estimated by LTB₄EIA kit. Data are expressed as Means±S.E of three experiments (^*; p<0.05 vs. control, ^#; p<0.05, ^##; p<0.01 vs. H₂O₂ alone).