Fig. 5.

The effect of eupatilin, SB202190, SP600125, NAC on p38 MAPK (A) and (B) JNK phosphorylation in EECS. Serum-starved EECs were preincubated in the presence of eupatilin (150 µM, 12 hr), SB202190 (p38 MAPK inhibitor, 30 µM, 1 hr), SP600125 (JNK inhibitor, 30 µM, 1hr), or NAC (N-acetyl-L-cysteine, ROS scavenger, 5 mM, 1 hr). EECs were then stimulated with H₂O₂ (300 µM, 24 hr). The change of phosphorylated p38MAPK and JNK was estimated by Western blot analysis. Data are expressed as Means±S.E of three experiments (^#; p<0.05 vs. control, ^*; p<0.05 vs. H₂O₂ alone).