Fig. 3.

Resveratrol down-regulates Bcl-2 family proteins. (A) B103 cells were cultured in 60-mm culture dishes to near 90% confluence and then starved in DMEM containing 0.5% FBS for 24 h. After 24 h starvation cells were treated with 0 to 20 µM of resveratrol. Whole cell lysates were subjected to 15% SDS-PAGE and the levels of Bcl-2, Bcl-xL, and Mcl-1 were detected by western blotting as described in materials and methods. β-actin was used as a loading control. (B~D) The intensities of the Bcl-2 bands, the Bcl-xL bands and the Mcl-1 bands were determined by densitometric scanning and analyzed by Bio-Profil software and the expression levels were normalized to β-actin. Results are mean±S.E. and representatives of three independent experiments are shown (n=3, ^*p<0.05, ^**p<0.01, ^***p<0.001).