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Interaction with RYR2 Peptide
Calmodulin binding to the RYR2-CBD peptide (Arg3581–Leu3611). The RYR2 Trp3586 fluorescence-emission spectrum, normalized to the maximal fluorescence intensity of the peptide alone, is shown without added calmodulin (RYR) and with a saturating amount of the indicated calmodulin variants added at (A) low intracellular (100 nM free Ca2+), (B) moderate (1 μM free Ca2+), and (C) saturating (200 μM Ca2+) concentrations.
