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. 2012 Oct 5;91(4):737–743. doi: 10.1016/j.ajhg.2012.08.020

Figure 2.

Figure 2

Steady-State Levels of Mitochondrial DNA, mRNAs, rRNAs, and Mitochondrial-Translation Proteins

(A) Southern blot analysis of genomic DNA extracted from control and subject fibroblasts. Hybridization was performed with probes directed against a 16 kb fragment of the mitochondrial genome, and the nuclear 18S rRNA gene was used as a loading control.

(B) Northern blot analysis carried out with total RNA extracted from control and subject fibroblasts. Hybridization was performed with probes specific to mitochondrial mRNAs encoding the three COX subunits, one of the complex I subunits (ND1), and the 12S and 16S mitochondrial ribosomal RNAs. Beta-actin was used as the loading control.

(C) Immunoblot analysis of control and subject fibroblasts with antibodies against the mitochondrial-translation elongation factors (EFG1and EFTs) and the mitochondrial ribosomal proteins MRPL32 (a kind gift of T. Langer, Cologne), MRPL13, MRPL15, and MRPS2 (kind gifts of L. Spremulli, UNC Chapel Hill). The 70 kDa subunit of complex II (SDHA) was used as a loading control.