Figure 1 .

Identification of AOX-deficient strains in the knockout library. (A) Conidial suspensions from strains known to lack AOX (aod strains), slow-growing sETC mutants (cya-5 and [mi-3]), and a control wild-type strain (NCN251) were plated on medium with or without antimycin A (+Ant A or –Ant A, respectively). Plates were incubated at 30° for 2 (−Ant A) or 4 (+Ant A) d and then photographed. (B) Plate number 1 of the knockout library contained strains of opposite mating type in which the aod-5 gene had been knocked out. Conidia from this knockout library plate were stamped onto medium with and without antimycin A. The boxes indicate the position of the aod-5 strains at positions C3 and C4.