Figure 2 .

AOX levels in knockout mutants identified in the screen. The indicated strains were grown for 24 and 48 hr in the presence of Cm. Mycelia were harvested, mitochondria were isolated, and mitochondrial proteins were subjected to SDS-PAGE and Western blot analysis using antibody to AOX, and to Tom70 as a loading control. Results are placed in rows A through H to facilitate reference to results for individual strains. The control (NCN251) was grown in similar fashion. A culture of the control without Cm also was used to demonstrate the lack of AOX without induction by Cm. AOX-deficient strains are shown with a control from the same Western blot. In some cases, irrelevant lanes were removed from the blots and are shown as white strips between lanes.