Table 2. Strains in which the gene knockout is predicted to encode a mitochondrially localized protein.

Strain	Mutant Class	NCU Number	Localization Criteriaa	Predicted Proteinb
40E6	1	05600	A2	Ubiquinone biosynthesis
96H9	1	07953	C1	Alternative oxidase (AOX)
2A3	2	08741	B1	Striatin pro 11
96C10	2	04245	A1	Tom70 protein import receptor
96G8	2	06727	C1	Spermidine 3, spermidine synthase
9E5	3	08565	B1	Hypothetical protein
13C11	3	06493	D2	Guanine nucleotide binding protein alpha-1
28B10	3	08137	C1	Hypothetical [BLAST yeast Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36]
82B6	3	07112	A1	Hypothetical [HcaD domain conserved many organisms, NAD(FAD) oxidoreductase]
101D3	3	09560	A1	Superoxide dismutase
103G4	3	09553	A1	3-hydroxybutyryl-CoA dehydrogenase
104D1	3	02973	C1	Mitochondrial carrier protein
104E9	3	01553	C1	Para-hydroxybenzoate-polyprenyltransferase Coq2

^aAs defined previously (Keeping et al. 2011). Category A1 proteins were identified in their study from highly purified mitochondria using mass spectrometry. Category A2 proteins were identified by mass spectrometry in other studies or had other evidence of being located in mitochondria. B1 proteins were identified in an earlier study of the mitochondrial proteome (Schmitt et al. 2006) but no other evidence for or against their mitochondrial location exists. C1 proteins were predicted or demonstrated to be mitochondrial by conventional biochemistry. The D2 proteins are listed in MitoP2 Neurospora data, but other evidence that they are not mitochondrial also exists. This refers to a mitochondrial proteome database for several organisms, including Neurospora (http://www.mitop.de/).

^bPrediction from the N. crassa database. Descriptions in brackets after the first description are taken from Keeping et al. (Keeping et al. 2011).