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. 2012 Nov 1;2(11):1379–1391. doi: 10.1534/g3.112.004093

Figure 5 .

Figure 5 

The MAGUK family member CG6509 regulates border cell cluster morphology. (A) Schematic of the CG6509 transcripts, which differ only in the 5′ non-coding exons (adapted from FlyBase); coding exons in orange. RNAi target sequences are indicated. (B) Schematic of CG6509 protein showing the conserved domains. (C) Quantification of border cell migration at stage 10, shown as the percentage of border cells with complete (green) or incomplete (red) migration in egg chambers expressing multiple RNAi lines or overexpression of full-length UAS-CG6509 (different insertions of same transgene) driven by c306-GAL4. Error bars represent SEM; n ≥ 50 egg chambers in each of at least three trials (*P < 0.05; **P < 0.01; two-tailed unpaired t-test). (D) Stage 9 border cells stained for GFP (green) and Singed (red) to reveal border cell cluster morphology. Representative example of a control (c306-GAL4/+; UAS-mCD8:GFP/+) border cell cluster. Two examples of CG6509 RNAi (c306-GAL4/+; UAS-mCD8:GFP/UAS-CG6509 RNAi v22496) border cells in which the cluster is partially dissociated (middle panel) or elongated (right panel). Scale bar is 20 μm.