Table 2. Expression levels and RNAi knockdown efficiency as measured by quantitative RT-PCR.
| Gene | Expression Level in the Ovary (CT value ± SD)a,b | RNAi Linec | Percentage Knockdown in Whole Fliesd |
|---|---|---|---|
| Positive candidates | |||
| baz | 25.7 ± 0.350 (L–M) | v2914 | 41*** |
| bbg | 27.4 ± 0.435 (L–M) | v15975 | 89** |
| CG6498 | 26.0 ± 0.640 (L–M) | v35100 | 53* |
| CG6509 | 26.3 ± 0.868 (L–M) | v22496 | 77** |
| Negative candidates | |||
| CG43707 | 34.5 ± 0.885 (L) | v25846 | 40 (ns) |
| CG43955 | 40.1 ± 1.51 (NE) | v103267 | 78** |
| CG9588 | 24.3 ± 0.318 (M–H) | HM05013 | 82*** |
| cnk | 25.5 ± 0.451 (L–M) | HMS00238 | 67*** |
| dlg1 | 25.4 ± 0.575 (L–M) | HMS01521 | 52** |
| dysc | 28.6 ± 0.237 (L–M) | v23278 | 32* |
| Grip | 28.4 ± 0.671 (L–M) | v103551 | 90*** |
| PICK1 | 30.4 ± 0.252 (L) | JF01199 | 21** |
| RHOGAP100F | 31.9 ± 0.874 (L) | HMS00740 | 14 (ns) |
| scrib | 25.8 ± 0.512 (L–M) | v105412 | 50** |
| sif | 31.2 ± 0.0700 (L) | v106832 | 16 (ns) |
| Syn1 | 31.2 ± 0.500 (L) | JF02654 | 32 (ns) |
| vari | 25.7 ± 0.0985 (L–M) | HM05087 | 72*** |
| X11L | 26.0 ± 0.463 (L–M) | v28652 | 55** |
Mean CT value and SD are calculated from three independent qPCR experiments. Expression summary: CT < 20, very high (VH); CT = 20–25, moderate to high (M–H); CT = 25–30, low to moderate (L–M); CT = 30–35, low (L); CT = 35–40, none to low (N–L); CT > 40, not expressed (NE).
For reference, the endogenous expression levels (mean CT value ± SD) of rp49 and tub84b were 17.7 ± 0.112 and 19.1 ± 0.16, respectively.
RNAi lines are from the Vienna Drosophila RNAi Center (prefixed with v) and from the Harvard Transgenic RNAi Project (prefixed with HM, HMS, or JF).
Extent of reduction of target gene expression compared with gfp dsRNA control, calculated using the ΔΔCT method with rp49 expression as reference. The one-tailed unpaired t-test was used to test for significance (ns, P > 0.05; *P = 0.01–0.05; **P = 0.001–0.01; ***P < 0.001).