Figure 1 .

Male flies trained with mated female trainer flies show reduced courtship toward mated female tester flies compared with their naïve counterparts at both 24 and 48 hr after training. Data are presented as box plots comparing courtship and wing extension indices between naïve and trained male flies. Lower and upper box borders denote the 25th and 75th percentiles, respectively. Middle lines denote the medians. Whiskers denote the most extreme data and outliers are plotted as individual dots. (A) Subsets of naïve and trained males removed from the same group of flies on which we performed transcriptome sequencing at 24 hr after training. In this experiment, n = 16 and 17 for naïve and trained flies respectively; ***P < 0.001 in Mann-Whitney U-test. (B) In a separate behavioral experiment, male flies were tested at both 24 and 48 hr after training. Reduced courtship at both time points indicates long-term persistence of courtship conditioning. In this experiment, n = 20 and 21 for naïve and trained flies, respectively, at 24 hr and n = 20 for both naïve and trained flies at 48 hr; **P < 0.05 in Mann-Whitney U-test.