Figure 1 .

Rbf1 exhibits dynamic promoter occupancy. Rbf1 occupancy of regulated promoters measured by ChIP was low in 0- to 6-hr embryos and peaked at 12 to 18 hr. Formaldehyde cross-linked chromatin was prepared from embryos of different ages and immunoprecipitated using the indicated antibodies. No specific enrichment was found at a nontarget gene promoter (sloppy paired 1). No Ab indicates immunoprecipitation carried out without antibody; IgG, nonspecific mouse polyclonal antibodies; α-H3, antihistone H3 antibody; and α-Rbf1, rabbit anti-Rbf1 antibody.