Table 1. Location of substitution(s) in nifA.
Plasmid | Location of residues (substitutions) in NifA* |
---|---|
First screening: | |
pMNA-2-1 | M173I (ATG→ATT), I196F, I217I, L418L, T516M |
pMNA-5-1 | No mutation |
pMNA-11-1 and 19-1 | M173V (ATG→GTG), D126Y, K208R, E413E |
pMNA-12-1 | M173V (ATG→GTG), V48G, T64S, R156H, P282T, E324A, R438H, G524D |
pMNA-14-5 and 23-4 | L41I, V96I, V132M, S241P, A360A, A376V, V473A |
pMNA-24-1 | M173I (ATG→ATA), P116L, A482V, M428L |
pMNA-26-4 | D126D, P146P |
Second screening: | |
pMNA-2-2 | V96A, S183R |
pMNA-3-2 | M173V (ATG→GTG), S16P, A376A |
pMNA-4-1 and 7-1 | M173I (ATG→ATT), A200A, R481F |
pMNA-8-6 | M173I (ATG→ATC), Y380Y |
pMNA-9-2 | M173I (ATG→ATT), V75A, K275K |
pMNA-10-1 | S32T, L486P |
pMNA-12-2 | E321N, F329F |
pMNA-14-4 and 26-3 | M173V (ATG→GTG), S22S, A243T, P511T |
pMNA-15-4 and 16-4 | M173I (ATG→ATA) |
pMNA-17-5 | M173I (ATG→ATA), T370A |
pMNA-18-2 | M173V (ATG→GTG), G126N, P207L |
pMNA-20-3 | M173I (ATG→ATT), Y522C |
pMNA-24-2 | M173V (ATG→GTG) |
pMNA-27-1 | M173I (ATG→ATT), K591N |
pMNA-28-1 | M173I (ATG→ATT), V86A |
pMNA-30-1 | M173I (ATGAATC), Y380Y, G556V |
The substitutions and codon changes at the M173 residue are shown in bold type. Base substitutions that did not change the encoded amino acid are also indicated (e.g. I217I).