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. Author manuscript; available in PMC: 2012 Oct 31.
Published in final edited form as: Microbiology (Reading). 2008 Sep;154(Pt 9):2689–2699. doi: 10.1099/mic.0.2008/019406-0

Table 2. Nitrogenase activity in the ΔglnB or the ΔglnD mutant with wild-type or altered nifA integrated into the chromosome when grown in MG (nitrogen-limiting) medium.

UR strain nifA integrated into DglnB or DglnD background Nitrogenase activity*
UR1739 Wild-type nifA integrated into ΔglnB mutant (UR717) <10
UR1740 nifA11 (encoding NifA-M173I) integrated into ΔglnB mutant (UR717) 470
UR1741 nifA12 (encoding NifA-M173V) integrated into ΔglnB mutant (UR717) 450
UR1742 Wild-type nifA integrated into ΔglnD mutant (UR1325) <10
UR1743 nifA11 (encoding NifA-M173I) integrated into ΔglnD mutant (UR1325) 200
UR1744 nifA12 (encoding NifA-M173V) integrated into ΔglnD mutant (UR1325) 230
*

Each unit of nitrogenase activity is expressed as nanomoles ethylene produced per hour per millilitre of cells at an OD600 of 1. Each activity value is from at least five replicate assays from different individually grown cultures. SD, 5–15 %.