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. Author manuscript; available in PMC: 2012 Oct 31.
Published in final edited form as: Microbiology (Reading). 2008 Sep;154(Pt 9):2689–2699. doi: 10.1099/mic.0.2008/019406-0

Table 3. Nitrogenase activity in R. rubrum wild-type or draT mutant with wild-type or altered nifA integrated into the chromosome when grown in MG (nitrogen-limiting) and MN (NH4+-excess) media.

Strain nifA integrated into wild-type or draT background Nitrogenase activity*

MG (nitrogen-limiting) MN (NH4+ -excess)
UR2 Wild-type 800 <10
UR2501 Wild-type with wild-type nifA 750 <10
UR2502 Wild-type with nifA11 (encoding NifA-M173I) 660 30
UR2503 Wild-type with nifA12 (encoding NifA-M173V) 770 30
UR2327 draT mutant with wild-type nifA 610 <10
UR2328 draT mutant with nifA11 (encoding NifA-M173I) 660 450
UR2329 draT mutant with nifA12 (encoding NifA-M173V) 500 540
*

Units of nitrogenase activity are expressed as in Table 2. Each activity value is from at least five replicate assays from different individually grown cultures. SD, 5–15 %.