. Author manuscript; available in PMC: 2012 Oct 31.

Published in final edited form as: Microbiology (Reading). 2008 Sep;154(Pt 9):2689–2699. doi: 10.1099/mic.0.2008/019406-0

Table 3. Nitrogenase activity in R. rubrum wild-type or draT mutant with wild-type or altered nifA integrated into the chromosome when grown in MG (nitrogen-limiting) and MN (NH₄⁺-excess) media.

Strain	nifA integrated into wild-type or draT background	Nitrogenase activity^*

		MG (nitrogen-limiting)	MN (NH₄⁺ -excess)
UR2	Wild-type	800	<10
UR2501	Wild-type with wild-type nifA	750	<10
UR2502	Wild-type with nifA11 (encoding NifA-M173I)	660	30
UR2503	Wild-type with nifA12 (encoding NifA-M173V)	770	30
UR2327	draT mutant with wild-type nifA	610	<10
UR2328	draT mutant with nifA11 (encoding NifA-M173I)	660	450
UR2329	draT mutant with nifA12 (encoding NifA-M173V)	500	540

Units of nitrogenase activity are expressed as in Table 2. Each activity value is from at least five replicate assays from different individually grown cultures. SD, 5–15 %.

Table 3. Nitrogenase activity in R. rubrum wild-type or draT mutant with wild-type or altered nifA integrated into the chromosome when grown in MG (nitrogen-limiting) and MN (NH4+-excess) media.

Table 3. Nitrogenase activity in R. rubrum wild-type or draT mutant with wild-type or altered nifA integrated into the chromosome when grown in MG (nitrogen-limiting) and MN (NH₄⁺-excess) media.