Table 5. Loci of Tn5 insertion and substitutions in nifA in R. rubrum mutants.
Tn5 insertion locus* | ORF* | Substitution in nifA |
---|---|---|
4194344 (9)† | Between Rru_A3654 and Rru_A3654 | A243T (2)‡ |
40371 (3) | Rru_ B0039 | M173I (2) |
2738176 (5) | Rru_ A2353 | L184R (2) |
1929051 (3) | Rru_ A1638 | M173I |
1974362 (3) | Rru_A1681 | G36E |
1113948 (21) | Rru_ A0934 | M173I |
3073513 (1) | Rru_A2643 | G36E |
2087876 (1) | Rru_ A1795 | No mutation in nifA |
3405657 (1) | Rru_ A2956 | M173I |
3047404 (1) | Rru_A2619 | M173L |
3533303 (1) | Rru_ A3067 | M173V |
3336134 (1) | Rru_ A2892 | T38P |
1264071 (1) | Rru_ A1073 | T38P |
184684 (1) | Rru_A0158 | M173V |
The Tn5 insertion loci and its ORF are based on the R. rubrum genomic sequence at http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=19059.
The numbers in parentheses indicate the number of strains that were sequenced and had identical loci of Tn5 insertion.
The numbers in parentheses indicate the number of strains in which nifA was sequenced.