Table 5. Loci of Tn5 insertion and substitutions in nifA in R. rubrum mutants.

Tn5 insertion locus*	ORF*	Substitution in nifA
4194344 (9)†	Between Rru_A3654 and Rru_A3654	A243T (2)‡
40371 (3)	Rru_ B0039	M173I (2)
2738176 (5)	Rru_ A2353	L184R (2)
1929051 (3)	Rru_ A1638	M173I
1974362 (3)	Rru_A1681	G36E
1113948 (21)	Rru_ A0934	M173I
3073513 (1)	Rru_A2643	G36E
2087876 (1)	Rru_ A1795	No mutation in nifA
3405657 (1)	Rru_ A2956	M173I
3047404 (1)	Rru_A2619	M173L
3533303 (1)	Rru_ A3067	M173V
3336134 (1)	Rru_ A2892	T38P
1264071 (1)	Rru_ A1073	T38P
184684 (1)	Rru_A0158	M173V

^*The Tn5 insertion loci and its ORF are based on the R. rubrum genomic sequence at http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=19059.

^†The numbers in parentheses indicate the number of strains that were sequenced and had identical loci of Tn5 insertion.

^‡The numbers in parentheses indicate the number of strains in which nifA was sequenced.