Table 6. Nitrogenase activity in R. rubrum ΔglnB mutants with NifA variants when grown in MG (nitrogen-limiting) medium.
Strain | nifA integrated into ΔglnB mutant (UR717) | Nitrogenase activity* |
---|---|---|
UR2 | Wild-type | 800 |
UR1739 | Wild-type nifA integrated into ΔglnB mutant | <10 |
UR1740 | nifA11 (encoding NifA-M173I) integrated into ΔglnB mutant | 470 |
UR1741 | nifA12 (encoding NifA-M173V) integrated into ΔglnB mutant | 450 |
UR11742 | nifA23 (encoding NifA-M173L) integrated into ΔglnB mutant | 450 |
UR11743 | nifA24 (encoding NifA-L184R) integrated into ΔglnB mutant | 400 |
UR11744 | nifA25 (encoding NifA-A243T) integrated into ΔglnB mutant | 500 |
UR11745 | nifA26 (encoding NifA-G36E) integrated into ΔglnB mutant | 220 |
UR11746 | nifA27 (encoding NifA-T38P) integrated into ΔglnB mutant | 310 |
Units of nitrogenase activity are expressed as in Table 2.