Figure 1.

Multiplexed detection of both antigen and antibodies from a spiked blood sample. IgM specific to Salmonella typhi was detected in parallel with antigen specific to Plasmodium falciparum (malaria). Separate sample processing (specimen dilution and IgG removal) and two separate assay types were performed automatically on the same card. Dry reagents stored on the card were used to minimize user steps. This work was part of the DxBox, a project to perform sample-to-result identification of multiplexed fever-causing pathogens using a pneumatically actuated point-of-care device.