Figure 6.

Protein-dependent farnesylcysteine and geranylgeranylcysteine α-carboxyl methyltransferase activities. Product formation in the presence of 200 μm AFC, AGGC, or AGC was measured as a function of the amount of tobacco membrane protein in the 60-min assay at 30°C. Arrows indicate the positions of relevant methylated products and the origins at which samples were spotted before silica gel TLC. The plot shown was generated by subtracting the AGC background. The background is less obvious than in Figures 3–5 because the fluorograms shown represent relatively short exposures.