Antibody responses to linear V2 loop peptides decline over time. (A) Antibody responses to linear biotinylated peptides 1, 2, 3, 4, and 6 at visits 1, 8, and 9 by ELISA using plasma sample set C. Absorbance at 405 nm is shown on the y-axis. (B) ELISA using the same peptides and plasma of 20 placebos, set C. Plasma was used at a dilution of 1:100 and peptide aa sequences are shown in the right upper corner of (B). Samples were run in duplicate and are the average of two experiments.