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. 2012 Jun 26;5(6):804–811. doi: 10.1242/dmm.009712

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 3. — Marked glycogen accumulation is present in hepatocytes at 4, 12 and 16 months of age. (A–C) Paraffin-embedded, H&E-stained liver sections illustrate the typical vacuolated appearance of glycogen-filled hepatocytes at 4, 12 and 16 months. (D–F) In HRLM sections stained with PAS-Richardson’s stain, the glycogen is well preserved and appears light purple. Dense fibrosis is evident in F; fibroblasts stain light blue. Scale bars: 50 μm (A,B,F), 100 μm (C) and 30 μm (D,E).