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. 2012 May 31;5(6):823–833. doi: 10.1242/dmm.008557

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 7. — Haemin induces the release of IL-1α but not IL-1β in primary mixed glia. Graphs show the release of IL-1α and IL-1β in LPS-treated mouse primary mixed glial cells after haemin (30 μM; black bars) or vehicle (DMSO; white bars) for 1, 2, 4 and 6 hours. ATP (5 μM) stimulation for 1 hour was used as a positive control (striped bar). IL-1α release was significantly increased after 2, 4 and 6 hours of haem stimulation compared with vehicle (A). Haem stimulation had no effect on release of IL-1β (B). n=4, one-way ANOVA followed by Student’s t-test with Bonferroni’s correction; *P<0.05, **P<0.01, ***P<0.001. Western blot analysis revealed that haemin (30 μM) induced cleavage of pro-IL-1α (31 kDa) to the mature form of IL-1α (17 kDa) through a calpain-1-dependent mechanism, because release of mature IL-1α was blocked by the calpain-1 inhibitor MDL28170 (100 μM) (C).