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IF for DMRT7. WT (A,C) and SCCx43KO (B,D) on day 15 p.p. (A,B) and in adulthood (C,D) were compared. Results showed a higher number of DMRT7 immunopositive cells in pubertal WT testis compared with SCCx43KO mice (A,B). In adult WT males, a specific staining can be detected in nearly all spermatocytes (C), whereas only paraffin autofluorescence is seen in KO tubules (D). Insert in C: note paraffin autofluorescence in interstitial LCs in negative controls. Scale bars: 50 μm.
