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. 2012 Jun 14;5(6):895–913. doi: 10.1242/dmm.008649

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 6. — Relative gene expression of WT mice versus SCCx43KO mice after applying the calculated correction factors. Bars represent mean±s.d. mRNA levels of three independent qRT-PCR experiments. Calculations were realized determining Actb and Hsp90ab1 as housekeeping genes and applying the ΔΔC(t) method. The genes Sox3, Dazl, Cx43, Piwil2, Tex11, Ddx25, Cdkn2a and Sohlh1 were significantly downregulated (*P<0.05), whereas the downregulation of Stra8 and Dmrt6 was close to significant (x). The only significant upregulation was detected for Amh.