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. 2012 Jun 14;5(6):895–913. doi: 10.1242/dmm.008649

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 9. — IHC confirmed also the findings obtained by microarray analysis and qRT-PCR for Dazl (GCs). The occurrence of GC-specific DAZL protein revealed obvious differences between WT (A,C) and SCCx43KO (B,D) mice. The number of stained cells is drastically reduced in 8-day-old SCCx43KO mice (B), confirming results obtained by qRT-PCR. Note immunonegative spermatogonia in B (arrows). In adult mice, a clear immunoreaction for DAZL can be seen in spermatogonia and spermatocytes (C). By contrast, in adult SCCx43KO mice not even spermatogonia (arrows) show a DAZL immunostaining (D). Insert in A: representative negative control for DAZL experiments. Scale bars: (A,B) 50 μm; (C,D) 100 μm.