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Copurification of anionic xylem peroxidases
| Separation | Volume | Protein | Peroxidase Enrichment |
|---|---|---|---|
| mL | mg | -fold | |
| Extract | 2900 | 255 | 1.0 |
| Ultra-filtrate | 600 | 171 | 1.5 |
| G25-permeate | 780 | 166 | 1.5 |
| DEAE-Sepharose | 180 | 29 | 8.8 |
| Phenyl-Sepharose | 150 | 4.2 | 61 |
| Concanavalin A | 32 | 1.1 | 232 |
Xylem (275 g) from 2-year-old poplar trees was ground and extracted.
