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Molecular data of the Mono-Q-separated isoenzymes
| Isoenzyme | Apparent Molecular Mass | pI | Glycans |
|---|---|---|---|
| kD | pH | estimated no. | |
| PXP 1 | 50.3, 54.3 | 3.83 | 15 |
| PXP 2 | 49.1, 53.6 | 3.83 | 13 |
| PXP 3 | 46.9, 48.2 | 3.43, 3.53 | 11 |
| PXP 4 | 47.4, 48.5 | 3.43, 3.53 | 11 |
| PXP 5 | 46.5, 48.2 | 3.73 | 11 |
| PXP 6 | 53.4 | 3.13 | 13 |
The estimation of the glycan number was calculated from the amino acid hydrolysis assuming two residues of N-glucosamine per glycan, a 50% loss in the 20-h hydrolysis, and peroxidases of 300 amino acids.
