Table IV.
Amino acid composition of the purified isoenzymes
| Amino Acid | PXP 1 | PXP 2 | PXP 3 | PXP 4 | PXP 5 | PXP 6 |
|---|---|---|---|---|---|---|
| mol % | ||||||
| Asn/Asp | 14.904 | 14.887 | 14.809 | 14.923 | 15.546 | 14.809 |
| Thr | 8.194 | 8.907 | 9.053 | 8.959 | 8.837 | 8.097 |
| Ser | 10.741 | 11.383 | 9.946 | 10.092 | 8.816 | 10.276 |
| Gln/Glu | 7.168 | 8.242 | 8.078 | 8.243 | 8.099 | 7.639 |
| Pro | 4.776 | 4.234 | 4.412 | 4.254 | 4.527 | 4.880 |
| Gly | 8.296 | 8.382 | 7.313 | 7.714 | 6.859 | 8.745 |
| Ala | 9.175 | 9.378 | 9.350 | 9.271 | 8.935 | 9.691 |
| Cys | 2.176 | 1.886 | 2.469 | 2.272 | 2.273 | 1.911 |
| Val | 5.176 | 4.695 | 5.204 | 5.146 | 6.167 | 4.465 |
| Met | 0.979 | 0.965 | 0.950 | 0.929 | 0.953 | 1.068 |
| Ile | 5.318 | 2.755 | 3.590 | 3.497 | 3.826 | 4.533 |
| Leu | 9.804 | 10.654 | 9.905 | 9.834 | 9.765 | 9.852 |
| Tyr | 1.295 | 1.372 | 1.381 | 1.379 | 1.242 | 1.428 |
| Phe | 4.737 | 5.434 | 5.764 | 5.733 | 6.122 | 4.719 |
| His | 1.055 | 1.211 | 1.126 | 1.139 | 1.027 | 1.165 |
| Lys | 1.644 | 1.693 | 2.174 | 2.178 | 2.017 | 2.174 |
| Arg | 4.562 | 3.923 | 4.477 | 4.437 | 4.988 | 4.548 |
| Trp | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Sum | 100 | 100 | 100 | 100 | 100 | 100 |
| GlcN | 4.93 | 4.31 | 3.74 | 3.59 | 3.78 | 4.25 |
All isoenzymes were hydrolyzed and analyzed as described in Methods (60 pmol sample−1). N-Glucosamine (GlcN) content was derived from the 20-h hydrolysates. Corrections for degradation of Ser (10%) and Thr (5%) for the 20-h hydrolyses were performed.