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. Author manuscript; available in PMC: 2012 Oct 31.
Published in final edited form as: Methods Enzymol. 2011;500:151–163. doi: 10.1016/B978-0-12-385118-5.00009-8

Figure 9.1.

Figure 9.1

On-array protein expression and capture. Left: Plasmid DNA is mixed with BSA, BS3 cross-linker, and the anti-GST capture antibody and arrayed on the aminosilane-coated glass slide. After blocking, cell-free expression mix is applied to the slide, and during a temperature-programmed incubation, the proteins are produced and bind to the capture antibody. Captured proteins can be detected by detecting the GST tag using a monoclonal anti-GST antibody, an HRP-labeled anti-mouse antibody, and Cy3-tyramide (TSA) HRP substrate. Right: Sample NAPPA images for PicoGreen staining of spotted DNA and anti-GST staining for captured proteins.