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. 2012 Oct 25;7(10):e48248. doi: 10.1371/journal.pone.0048248

Figure 1. Gene construct encoding the 425scFv-Bs recombinant protein.

Figure 1

The 425scFv-Bs-His6 construct starts with an N-terminal short FLAG tag (F, dark blue) followed by 425scFv in VH-linker-VL orientation (VH, cyan; L, gray; VL, turquoise), 16-amino-acid hinge linker (green), barstar (purple). The construct terminates with a His6-tag (dark blue) attached to the C-terminus of 425scFv-barstar fusion protein. The fusion gene is under control of the lac promoter. OmpA – the signal peptide for directed secretion of the recombinant protein to the E. coli periplasm.