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. 2012 Oct 25;7(10):e48248. doi: 10.1371/journal.pone.0048248

Figure 3. Purification and characterization of fusion proteins.

Figure 3

(A) 12% SDS-PAGE confirming the purification of (4D5scFv)2-Bn (a, 71 kDa) and 425scFv-Bs (a', 40 kDa), Coomassie Brillian Blue R-25 stained gel; standard protein marker (M) and fusion protein lanes (P) are shown. (B) RNAse activity of (4D5scFv)2-Bn (solid line) compared with activity of free barnase (dashed line) and evaluated with acid-insoluble RNA precipitation assay. (C) Inhibition of free barnase by 425scFv-Bs (solid line) compared with inhibition by free barstar (dashed line).