Table 3. Cycle threshold (Ct) values of Crh and Ucn1 mRNA in rat fetal tissues.
Organ | Crh Average Ct (SD) | Ucn1 Average Ct (SD) | Gestational Day |
Adrenal gland | ND | ND | 19 |
Bladder | ND | ND | 19 |
Brain | 30.1 (0.01) | ND | 19 |
Epididymis | ND | ND | 19 |
Gubernaculum | ND | ND | 19 |
Heart | ND | ND | 19 |
Hypothalamus | 32.1 (0.96) | ND | 17 |
Intestine | ND | ND | 19 |
Kidney | ND | ND | 19 |
Liver | ND | ND | 19 |
Lung | ND | ND | 19 |
Pancreas | ND | ND | 19 |
Placenta | ND | ND | 15 |
Placenta | ND | ND | 17 |
Placenta | ND | ND | 19 |
Spleen | ND | ND | 19 |
Stomach | ND | ND | 19 |
Testis | ND | ND | 15 |
Testis | ND | ND | 17 |
Testis | ND | ND | 19 |
ND = Not detectable; Ct value over 34. Applied Biosystems does not recommend using any data with a Ct value over 34 due to poor accuracy.