Table 2. Potential target mRNAs of identified miRNAs.
| Symbol | RefSeq ID | Name | miRNA |
| AKAP13 | NM_006738.4 | A kinase (PRKA) anchor protein 13 | miR-17, miR-20a, miR-93, miR-106a, miR-106b |
| ARHGAP24 | NM_001025616.1 | Rho GTPase activating protein 24 | miR-133a, miR-133b, miR-590-5p |
| CPEB3 | NM_014912.3 | cytoplasmic polyadenylation element binding protein 3 | miR-29a, miR-29b |
| IRS2 | NM_003749.2 | insulin receptor substrate 2 | ?miR-7, miR-181b |
| KCNJ10 | NM_002241.2 | potassium inwardly-rectifying channel, subfamily J, member 10 | miR-93, miR-106b |
| KIAA1632 | NM_020964.1 | KIAA1632 | miR-25, miR-32 |
| LIMK1 | NM_016735.1 | LIM domain kinase 1 | miR-20a, miR-20b, miR-106a |
| MEX3B | NM_032246.3 | mex-3 homolog B (C. elegans) | miR-29a, miR-29b |
| MPP1 | NM_002436.2 | membrane protein, palmitoylated 1, 55kDa | miR-32, miR-25 |
| MTF1 | NM_005955.1 | metal-regulatory transcription factor 1 | ?miR-25, miR-130b |
| PIK3R1 | NM_181504.2 | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) | miR-29a, miR-29b, miR-96 |
| PIK3R3 | NM_003629.2 | phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) | miR-21*, miR-29a, miR-29b |
| PTEN | NM_000314.3 | phosphatase and tensin homolog (mutated in multiple advanced cancers 1) | miR-17, miR-20b, miR-9*, miR-92a |
| RHOC | NM_175744.3 | ras homolog gene family, member C | miR-17, miR-20a, miR-20b, miR-106a |
| SAPS2 | XM_942540.1 | PREDICTED: SAPS domain family, member 2 | miR-20b, miR-106a |
| SLC25A37 | NM_016612.1 | solute carrier family 25, member 37 (SLC25A37) | miR-181b, miR-181d |
| SLC2A3 | NM_006931.1 | solute carrier family 2 (facilitated glucose transporter), member 3 | miR-195, miR-497 |
| SNX4 | NM_003794.2 | sorting nexin 4 | miR-29a, miR-29b |
| SOX5 | NM_006940.4 | SRY (sex determining region Y)-box 5 | miR-195, miR-497, miR-503 |
| SYT7 | NM_004200.2 | synaptotagmin VII | miR-17, miR-20a, miR-20b, miR-93, miR-106b |
| TGFBR2 | NM_001024847.1 | transforming growth factor, beta receptor II (70/80kDa) | miR-9, miR-590-5p |
| TNFRSF1B | NM_001066.2 | tumor necrosis factor receptor superfamily, member 1B | miR-19a, miR-19b, miR-148b, miR-301a, miR-301b |
| WDR40A | NM_015397.1 | WD repeat domain 40A | miR-29a, miR-29b |
| ZBTB47 | NM_145166.2 | zinc finger and BTB domain containing 47 | miR-7-1*, miR-17, miR-18a, miR-18b, miR-19a, miR-19b, miR-20a, miR-20b, miR-106a |
| ZFP91 | NM_170768.1 | zinc finger protein 91 homolog (mouse) | ?miR-17, miR-19a, miR-19b, ?miR-20a, ?miR-20b |
| ZNF385D | NM_024697.1 | zinc finger protein 385D | miR-32, miR-92a |
∧
miRNA with more than one binding site for the mRNA.
The mRNAs are differently expressed in osteosarcoma cell lines and clinical samples and predicted to be targeted by several of the differently expressed miRNAs. The pairs of miRNAs and mRNA show a Pearson’s correlation (r<−0.5) in cell lines.