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. 2012 Oct 25;7(10):e47726. doi: 10.1371/journal.pone.0047726

Figure 2. Complementation of a yeast inorganic phosphate (Pi) transport mutant by GmPTs genes.

Figure 2

Yeast MB192 cells harboring either an empty expression vector (control) or the candidate Pht1 ORF(open reading frame), transformants were grown in YNB medium to an OD600 ≈0.8, then washed by 3% glucose with centrifugation at 1500 g, 4°C, and suspended in phosphate free YNB medium to OD600 ≈1.0. Different number cells (5×105, 5×104, 5×103) were applied to Pi-limiting medium (20 µM, pH 6.0) then incubated at 30°C for 3 d.