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. 2012 Oct 25;7(10):e47726. doi: 10.1371/journal.pone.0047726

Table 2. Kinetic parameter estimates of GmPTs-mediated inorganic phosphate (Pi) transport.

Yeast Km(µM) Vmax(µM/g [dry weight]·min)
Yp112-GmPT1 67.30±15.60 298.30±24.40
Yp112-GmPT2 44.00±12.90 295.00±20.90
Yp112-GmPT3 30.00±8.60 226.70±16.30
Yp112-GmPT4 65.30±13.30 282.30±43.28
Yp112-GmPT5 25.70±1.63 231.30±18.80
Yp112-GmPT6 153.00±44.00 281.70±37.60
Yp112-GmPT7 105.30±20.00 247.00±24.50
Yp112-GmPT8 46.00±5.60 231.50±5.50
Yp112-GmPT9 88.00±8.90 240.30±7.10
Yp112-GmPT10 231.00±15.60 400.00±25.00
Yp112-GmPT11 79.00±8.30 271.30±45.00
Yp112-GmPT12 45.30±14.53 245.30±15.60
Yp112-GmPT13 32.30±1.60 265.70±32.50
Yp112-GmPT14 116.30±10.00 373.00±14.10
Yp112 127.00±8.50 154.70±3.60

Km and Vmax for yeast strain MB192 expressing the indicated GmPTs or carrying the empty expression vector (control) were determined at pH 6.0. The GmPTs mediated 33Pi uptake velocities, calculated according to their total Pi transport, following the Michaelis-Menten kinetics equation. Values shown are means ± SE for three independent experiments.