Table 2. Kinetic parameter estimates of GmPTs-mediated inorganic phosphate (Pi) transport.
Yeast | Km(µM) | Vmax(µM/g [dry weight]·min) |
Yp112-GmPT1 | 67.30±15.60 | 298.30±24.40 |
Yp112-GmPT2 | 44.00±12.90 | 295.00±20.90 |
Yp112-GmPT3 | 30.00±8.60 | 226.70±16.30 |
Yp112-GmPT4 | 65.30±13.30 | 282.30±43.28 |
Yp112-GmPT5 | 25.70±1.63 | 231.30±18.80 |
Yp112-GmPT6 | 153.00±44.00 | 281.70±37.60 |
Yp112-GmPT7 | 105.30±20.00 | 247.00±24.50 |
Yp112-GmPT8 | 46.00±5.60 | 231.50±5.50 |
Yp112-GmPT9 | 88.00±8.90 | 240.30±7.10 |
Yp112-GmPT10 | 231.00±15.60 | 400.00±25.00 |
Yp112-GmPT11 | 79.00±8.30 | 271.30±45.00 |
Yp112-GmPT12 | 45.30±14.53 | 245.30±15.60 |
Yp112-GmPT13 | 32.30±1.60 | 265.70±32.50 |
Yp112-GmPT14 | 116.30±10.00 | 373.00±14.10 |
Yp112 | 127.00±8.50 | 154.70±3.60 |
Km and Vmax for yeast strain MB192 expressing the indicated GmPTs or carrying the empty expression vector (control) were determined at pH 6.0. The GmPTs mediated 33Pi uptake velocities, calculated according to their total Pi transport, following the Michaelis-Menten kinetics equation. Values shown are means ± SE for three independent experiments.