Table 2. C. riparius transcriptome sequencing and assembly statistics.
| Category | Sequences | Base pairs |
| Sequencing statistics | ||
| Raw NGS reads | 1,549,146 | 500,673,325 |
| NGS reads 1 | 1,540,849 | 459,548,838 |
| NGS reads N50 2 | – | 347 |
| Assembly statistics | ||
| Assembled NGS reads | 1,342,920 | 409,774,142 |
| Discarded NGS reads 3 | 63,401 | 10,253,972 |
| Singletons | 135,082 | 39,520,724 |
| Singleton N50 2 | – | 343 |
| Contigs ("exons") | 27,334 | 21,898,252 |
| Contig N50 2 | – | 1,161 |
| Mean # NGS reads/contig | 59.0 | – |
| Isotigs ("transcripts") | 23,683 | 32,429,684 |
| Isotig N50 2 | – | 1,886 |
| Mean # contigs/isotig | 1.9 | – |
| Isogroups ("genes") | 18,514 | – |
| Mean # isotigs/isogroup | 1.3 | – |
1
after trimming of adaptor sequences;
2
N50 is a weighted median, such that half the bases are contained in sequences equal to or larger than the N50 length;
3
NGS reads that were discarded during the assembly process because they were too short (<50 bp), contained repeats or were marked as outliers by the Newbler assembler.