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. 2012 Oct 25;7(10):e47796. doi: 10.1371/journal.pone.0047796

Figure 5. Gatifloxacin Causes activation of p53 and inhibits pAKT.

Figure 5

(A) (i) Real Time PCR analysis of p53 expression in MIA PaCa-2 and Panc-1 cells treated with Gatifloxacin in a dose dependent manner, (ii) Real Time PCR analysis of p53 expression in MIA PaCa-2 and Panc-1 cells treated with 200µg/ml of Gatifloxacin in a time dependent manner. 18S rRNA was used to normalize the results. (B) (i) Western blot analysis of p53, AKT and pAKT expression in MIA PaCa-2 and Panc-1 cells treated with Gatifloxacin in a dose dependent manner, (ii) Western blot analysis of p53, AKT and pAKT expression in MIA PaCa-2 and Panc-1 cells treated with Gatifloxacin in a time dependent manner. β-actin was used as loading control. Data are representative of typical experiment repeated three times with similar results. Bar Graph represents the mean ± SEM. *p<0.01, #p<0.05 compared to control.