(A) HPLC chromatograms of glucosinolate profiling in laser-microdissected samples from rapeseed detected at 229 nm. ▾ contamination peaks. (B) Total glucosinolate concentration and concentrations of individual glucosinolates 1–11 in four dissected samples. HR, hypocotyl and radicle; IC, inner cotyledon; OC, outer cotyledon; and SE, seed coat and endosperm. Each column shows the mean of four replicates with standard error. *means not detectable. Peaks: 1, progoitrin; 2, epiprogoitrin; 3, glucoraphanin; 4, gluconapoleiferin; 5, glucoalyssin; 6, gluconapin; 7, 4-hydroxyglucobrassicin; 8, glucobrassicanapin; 9, glucoerucin; 10, glucoberteroin; and 11, gluconasturtiin.