Figure 4. Zn²⁺ induces a large current in FLCs.

(A) Membrane potentials were recorded in current clamp configuration. Zn²⁺ induced rapid membrane depolarization in FLCs cultured from wild-type mice, but not Gpr39 ^−/− cells. (B) Bar chart summarizing Zn²⁺ induced membrane depolarization in FLCs as in panel A. p<0.01. (C and D) Zn²⁺ induced a large current in wild-type. Current was sampled at +80 and −80 mV. (C) shows a time-series plot for a typical Zn²⁺-induced current in FLCs (n = 21). (D) shows the corresponding I-V relationship. (E and F) are similar experiments using FLCs isolated from GPR39 knockout mice and show that no Zn²⁺-induced current was observed in Gpr39 ^−/− FLCs (n = 11).