Figure 4. Western blot analysis of V-H⁺-ATPase subunit E protein expression in response to NaCl stress.

Immunostaining was performed using rabbit anti-VHA-E antibody. Western blots of tonoplast vesicles from roots (A) and leaves (B) are presented. Subunit E protein levels in the roots (C) and leaves (D) were quantified. The protein level of subunit E in the control was set to 100, and the protein levels of NaCl stressed plants were compared with control.