Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Gastroenterology. 2012 May 17;143(3):821–31.e1-2. doi: 10.1053/j.gastro.2012.05.009

Figure 2. Impaired exocrine pancreas regeneration in Bmi1 KO mice after caerulein pancreatitis.

Figure 2

Control or Bmi1 KO mice were injected with caerulein and sacrificed 7 days post-injection.

(A) Macroscopic views of pancreas (outlined with dashed lines), showing hypoplastic pancreas in Bmi1 KO mice (red) compared to control mice (blue).

(B) H&E staining showing impaired exocrine pancreas regeneration with increased duct-like structures and reduced acinar area in Bmi1 KO pancreas compared to control mice.

(C) Co-staining for amylase/CK19/DAPI reveals reduced number of amylase-positive cells and increased number of CK19-positive duct-like epithelial cells in Bmi1 KO pancreas.

(D) Immunostaining for Hes1, Sox9, and Pdx1. Immunohistochemistry shows Hes1 expression in the duct-like epithelial cells in Bmi1 KO pancreas 7 days post-injection. Co-staining for Sox9/DAPI demonstrates Sox9 expression in the duct-like epithelial cells in Bmi1 KO pancreas 7 days post-injection. Immunohistochemistry shows absence of Pdx1 expression in the duct-like epithelial cells in Bmi1 KO pancreas 7 days post-injection. Pdx1 remains expressed in control and mutant islets.

(E) Relative expression levels of Sox9 and Hes1 in control and Bmi1 KO pancreata 7 days post-injection by q-PCR. N = 3 mice.

(F) Relative expression levels of Ptf1a and Mist1 in control and Bmi1 KO pancreata 3 days post-injection. N = 3 mice. Means ± SD. * p< 0.05.