Figure 4. Increased apoptosis and decreased cell proliferation in Bmi1 KO pancreas after caerulein pancreatitis.
(A) Co-staining for cleaved Caspase 3 (c-Caspase 3)/E-cadherin/DAPI in control and Bmi1 KO pancreata 3 days post caerulein injection.
(B) Co-staining for TUNEL/E-cadherin/DAPI in contro l and Bmi1 KO pancreata 3 days post caerulein injection.
(C) Quantification of the number of Cleaved Caspase 3/E-cadherin double positive cells per field in control and Bmi1 KO pancreata 3 days and 7 days post-injection. N = 3 mice.
(D) Co-staining for phospho-histone H3 (PHH3)/E-cadherin/DAPI in control and Bmi1 KO pancreata 3 days post caerulein injection.
(E) Quantification of the number of PHH3/E-cadherin double positive cells per field in control and Bmi1 KO pancreata 3 days and 7 days post caerulein injection. N = 3 mice. Means ± SD. ** p < 0.01.