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. 2012 Oct 24;122(11):3990–4002. doi: 10.1172/JCI65508

Figure 5. CISTR-ACT in rodents.

Figure 5

(A and B) Phylogenetic analysis of the CISTR-ACT DNA and lncRNA sequences. 121 conserved TFBSs were bioinformatically predicted. The differences of the scales indicate that the lncRNAs are evolutionarily more divergent than the conserved DNA sequences. (C) Genomic arrangement of murine and rat Pthlh, Sox9, and CISTR-ACT–encoded lncRNAs AW491522 and BM385392, respectively, suggested an in trans network. (D) siRNA transfections of mouse ATDC5 (10 days differentiated) chondrocytes. Depletion of the mouse lncRNA AW491522 caused Pthlh or Sox9 downregulation. Knockdown of either Pthlh or Sox9 depleted the lncRNA (n = 4). (E) siRNA assays in RCSs. An analogous expression-dependent network was observed compared with the ATDC5 results (n = 5).**P ≤ 0.01.