Table 1.
Open reading frames encoded by Tn 6164
| Gene | Position on Tn 6164 | Module | Sequence identity to | Annotation | Gene | Position on Tn 6164 | Module | Sequence identity to | Annotation |
|---|---|---|---|---|---|---|---|---|---|
|
Orf1 |
650-1930 |
A |
- |
putative modification methylase |
Orf25 |
26793-27122 |
B |
- |
conserved hypothetical protein |
|
Orf2 |
1915-3186 |
A |
- |
putative modification methylase |
Orf26 |
27189-28451 |
B |
Thermoanaerobacter sp. |
HK97 family phage portal protein |
|
Orf3 |
3252-3962 |
A |
- |
hypothetical protein |
Orf27 |
28448-29128 |
B |
Thermoanaerobacter sp. |
Peptidase S14, ClpP |
|
Orf4 |
3952-5031 |
A |
- |
ATPase associated with various cellular activities |
Orf28 |
29140-30339 |
B |
Thermoanaerobacter sp. |
HK97 family phage major capsid protein |
|
Orf5 |
5047-6312 |
A |
- |
LlaJI restriction endonuclease |
Orf29 |
30585-30899 |
B |
Thermoanaerobacter sp. |
uncharacterized phage protein |
|
Orf6 |
C 7557-6361 |
A |
- |
Protein with unknown function, contains a C-terminal CGNR Zinc finger motif |
Orf30 |
30903-31238 |
B |
Thermoanaerobacter sp. |
phage head-tail adaptor, putative |
|
Orf7 |
8000-8494 |
B |
Thermoanaerobacter sp. |
ECF RNA polymerase sigma-24 factor |
Orf31 |
31252-31662 |
B |
Thermoanaerobacter sp. |
HK97 family phage protein |
|
Orf8 |
8809-9126 |
B |
Thermoanaerobacter sp. |
rRNA biogenesis protein rrp5, putative |
Orf32 |
31659-32012 |
B |
Thermoanaerobacter sp. |
Protein of unknown function (DUF806); |
|
Orf9 |
9123-10250 |
B |
Thermoanaerobacter sp. |
Phage associated protein |
Orf33 |
32016-32618 |
B |
Thermoanaerobacter sp. |
DUF3647 Phage protein (HHPred) |
|
Orf10 |
10256-10816 |
B |
Thermoanaerobacter sp. |
phage-associated protein |
Orf34 |
33330-35786 |
B |
Thermoanaerobacter sp. |
Phage tape measure protein |
|
Orf11 |
10813-12747 |
B |
Thermoanaerobacter sp. |
DNA-directed DNA polymerase |
Orf35 |
35800-36573 |
B |
Thermoanaerobacter sp. |
phage putative tail component |
|
Orf12 |
12795-13625 |
B |
Thermoanaerobacter sp. |
Prophage antirepressor |
Orf36 |
36692-39100 |
B |
Thermoanaerobacter sp. |
phage minor structural protein |
|
Orf13 |
13629-14048 |
B |
Thermoanaerobacter sp. |
DUF 4406 (HHPred) |
Orf37 |
39320-39901 |
B |
Thermoanaerobacter sp. |
Putative Sipho Phage tail protein (HHPred) |
|
Orf14 |
14045-16390 |
B |
Thermoanaerobacter sp. |
virulence-associated E protein |
Orf38 |
39928-42369 |
B |
Thermoanaerobacter sp. |
glycosyl hydrolase-like protein |
|
Orf15 |
16910-18259 |
B |
Thermoanaerobacter sp. |
SNF2-related protein |
Orf39 |
42430-42855 |
B |
Thermoanaerobacter sp. |
toxin secretion/phage lysis holin |
|
Orf16 |
18264-18722 |
B |
Thermoanaerobacter sp. |
phage-associated protein |
Orf40 |
42855-43556 |
B |
Thermoanaerobacter sp. |
N-acetylmuramoyl-L-alanine amidase |
|
Orf17 |
18842-19201 |
B |
Thermoanaerobacter sp. |
HNH endonuclease |
Orf41 |
43975-45540 |
B |
Thermoanaerobacter sp. |
phage integrase family site-specific recombinase/resolvase |
|
Orf18 |
19314-19865 |
B |
Thermoanaerobacter sp. |
Phage terminase, small subunit |
Orf42 |
45541-45954 |
B |
Thermoanaerobacter sp. |
recombinase/integrase |
|
Orf19 |
19883-21058 |
B |
Thermoanaerobacter sp. |
S-adenosylmethionine synthetase |
Orf43 |
46222-47529 |
B |
Thermoanaerobacter sp. |
phage integrase family site-specific recombinase |
|
Orf20 |
21039-22283 |
B |
Thermoanaerobacter sp. |
DNA methylase N-4/N-6 domain-containing protein |
Orf44 |
47987-48856 |
C |
E. faecalis pEF418 |
Nucleotidyl transferase |
|
Orf21 |
22384-23076 |
B |
Thermoanaerobacter sp. |
hypothetical/virulence-related protein |
Orf45 |
48837-49571 |
C |
E. faecalis pEF418 |
methyltransferase |
|
Orf22 |
23445-24344 |
B |
Thermoanaerobacter sp. |
Putative amidoligase enzyme |
Orf46 |
49604-50467 |
C |
E. faecalis pEF418 |
putative aminoglycoside 6-adenylyltansferase |
|
Orf23 |
24382-24843 |
B |
Thermoanaerobacter sp. |
AIG2/GGCT-like protein |
Orf47 |
50511-51038 |
C |
E. faecalis pEF418 |
putative adenine phosphoribosyltransferase |
|
Orf24 |
25462-26685 |
B |
Thermoanaerobacter sp. |
phage terminase |
Orf48 |
51251-51979 |
C |
E. faecalis pEF418 |
putative spectinomycin/streptomycin adenyltransferase |
|
Orf49 |
52403-53176 |
E |
S. pneumoniae |
phage protein/replication initiator |
Orf71 |
77648-79216 |
E |
S. pneumoniae |
putative surface protein |
|
Orf50 |
53176-54000 |
E |
S. pneumoniae |
DNA replication protein |
Orf72 |
79231-80088 |
E |
S. pneumoniae |
putative bacteriocin |
|
Orf51 |
53993-54478 |
E |
S. pneumoniae |
DUF 3801 |
Orf73 |
80162-80773 |
E |
S. pneumoniae |
Predicted transcriptional regulator |
|
Orf52 |
54475-55209 |
E |
S. pneumoniae |
phage antirepressor protein |
Orf74 |
80766-81749 |
E |
S. pneumoniae |
Protein with unknown function |
|
Orf53 |
55202-56890 |
E |
S. pneumoniae |
TraG/TraD family protein |
Orf75 |
82268-82621 |
E |
S. pneumoniae |
transcriptional regulator, ArsR family |
|
Orf54 |
57454-58486 |
E |
- |
DUF 318 Predicted Permease (HHPred) |
Orf76 |
82696-83940 |
E |
S. pneumoniae |
major facilitator superfamily MFS_1 |
|
Orf55 |
59048-59398 |
D |
C. fetus |
glyoxalase family protein |
Orf77 |
83927-84403 |
E |
S. pneumoniae |
toxin-antitoxin system, toxin component, GNAT domain protein |
|
Orf56 |
59411-59938 |
D |
C. fetus |
transcriptional regulator |
Orf78 |
84758-86491 |
E |
S. pneumoniae |
DNA topoisomerase III |
|
Orf57 |
59988-61910 |
D |
C. fetus |
tetracycline resistance protein |
Orf79 |
86484-87449 |
E |
S. pneumoniae |
possible DNA (cytosine-5-)-methyltransferase |
|
Orf58 |
62225-63082 |
D |
C. fetus |
aminoglycoside 6-adenylyltransferase (AAD(6) |
Orf80 |
87436-95079 |
E |
S. pneumoniae |
superfamily II DNA and RNA helicase |
|
Orf59 |
63575-64348 |
E |
S. pneumoniae |
replication initiator/phage |
Orf81 |
95123-95779 |
E |
S. pneumoniae |
putative single-stranded DNA binding protein |
|
Orf60 |
64345-65172 |
E |
S. pneumoniae |
replicative DNA helicase |
Orf82 |
95939-96841 |
E |
S. pneumoniae |
transcriptional regulator, XRE family |
|
Orf61 |
65314-65814 |
E |
S. pneumoniae |
TnpX site-specific recombinase family protein |
Orf83 |
97071-98282 |
E |
S. pneumoniae |
transporter, major facilitator family/multidrug resistance protein 2 |
|
Orf62 |
65938-66399 |
E |
S. pneumoniae |
flavodoxin |
Orf84 |
C 99739-98462 |
E |
S. pneumoniae |
relaxase/type IV secretory pathway protein VirD2 |
|
Orf63 |
66817-67302 |
E |
S. pneumoniae |
putative conjugative transposon protein |
Orf85 |
C 101169-99795 |
E |
S. pneumoniae |
conjugal transfer relaxosome component TraJ |
|
Orf64 |
67299-68033 |
E |
S. pneumoniae |
phage antirepressor protein |
Orf86 |
C 101403-100321 |
E |
S. pneumoniae |
toxin-antitoxin system, toxin component, Fic family |
|
Orf65 |
68026-69816 |
E |
S. pneumoniae |
TraG/TraD family protein/putative conjugal transfer protein |
Orf87 |
C 101878-101396 |
E |
S. pneumoniae |
putative membrane protein |
|
Orf66 |
70395-70706 |
E |
S. pneumoniae |
putative single-strand binding protein |
Orf88 |
C 102435-101887 |
E |
S. pneumoniae |
putative toxin-antitoxin system, toxin component |
|
Orf67 |
70934-71797 |
E |
S. pneumoniae |
conjugative transposon membrane protein |
Orf89 |
C 102845-102444 |
E |
S. pneumoniae |
regulator/toxin-antitoxin system, antitoxin component |
|
Orf68 |
72099-72509 |
E |
S. pneumoniae |
conjugative transposon membrane protein |
Orf90 |
103034-103555 |
E |
S. pneumoniae |
conserved hypothetical protein |
|
Orf69 |
72580-74823 |
E |
S. pneumoniae |
type IV conjugative transfer system protein |
Orf91 |
103825-104235 |
E |
S. pneumoniae |
sigma-70, region 4 |
| Orf70 | 74831-77410 | E | S. pneumoniae | conjugative transposon cell wall hydrolase/NlpC/P60 family | Orf92 | 104966-106712 | E | S. pneumoniae | site-specific recombinase, resolvase family |