Table 3.

Oligonucleotides used in this study

Name	Sequence	Purpose
1	GAGATATGGTTATGAGATTAGG	Presence/absence of insert
2	CCCACCTTTATAGCATCATATAG	Absence of insert
3	CTAACCTATCAACTCAACCCC	Presence of insert
4	AGGATAAGACCGCAGCAGAA	Presence 5’half of insert
5	AAAAACGACGGTTTTCCTGTG	Presence 5’half of insert
6	GGGCAAATAGAAAGTCAAAACG	Presence 3’half of insert
7	AAGTGGTGTTTTCTTTGGAGGA	Presence 3’half of insert
8	CCACAGGGATACCTTTCTCGTGC	Presence of tet(44) gene
9	TTCCATATCCTCGGGTTTTTGCAT	Presence of tet(44) gene
10	CAGGTGTTGAAATAGATATTGAG	Detect 3' end half insert
11	CAGAAGTCGATCCTTTCTGGG	Detect 3' end half insert
12	GGTGGCTGAACTCGTTAATC	Detect 3' end half insert
13	CTCCACATGGCTCGAGTTG	Detect 3' end half insert
14	GAGGAATTTAACAGAACAGTATTT	Excision studies
15	TCTATCCTGCCTTCTCAACC	Excision studies
16	CGAATCGCTGAAATGACTGA	Excision studies
17	GCGAATGATTTCATGGAAGG	Excision studies
18	CGACTGCATTACCAGTTCCA	Excision studies
Lok1 [13]	AAAATATACTGCACATCTGTATAC	Transconjugant screening
Lok3 [13]	TTTACCAGAAAAAGTAGCTTTAA	Transconjugant screening
19	CAGCTGCAGTTTTTCCATGA	Transconjugant screening
20	GCAGCTAACGGTGATGACAA	Transconjugant screening
Tn916 Fw [30]	GACGGAAGATACTTATACA	Transconjugant screening
Tn916 Rev [30]	GCCTTTGGATTCATTCCTGC	Transconjugant screening