Table 2.
Summary of selected temporal phosphoproteomic data
| Protein | Residue | Wolf-Yadlin et al. (2007) | VanMeter et al. (2009) | Ciaccio et al. (2010) |
|---|---|---|---|---|
| ERBB1 (EGFR) |
|
|
|
|
| |
Y845 |
|
|
X |
| |
Y992 |
|
X |
|
| |
Y998 |
X |
|
|
| |
Y1045 |
X |
|
|
| |
Y1068 |
|
X |
X |
| |
Y1086 |
|
|
X |
| |
Y1148 |
X |
X |
|
| |
Y1173 |
X |
X |
X |
| ERBB2 (HER2) |
|
|
|
|
| |
Y1221/1222 |
|
|
X |
| |
Y1248 |
X |
X |
|
| ERBB3 (HER3) |
|
|
|
|
| |
Y1289 |
|
|
X |
| |
Y1328 |
|
|
|
| ERBB4 |
|
|
|
|
| |
Y1284 |
|
|
X |
| Shc1 |
|
|
|
|
| |
Y239/240 |
X |
|
X |
| |
Y317 |
X |
X |
X |
| Raf-1 |
|
|
|
|
| |
S289/296/301 |
|
|
X |
| |
S338 |
|
|
X |
| MEK1/2 |
|
|
|
|
| |
S217/T221 |
|
|
X |
| ERK1/2 |
|
|
|
|
| |
T202/Y204 |
X |
X |
X |
| Gab1 |
|
|
|
|
| |
Y373 |
X |
|
|
| |
Y406 |
X |
|
|
| |
Y627 |
X |
X |
X |
| |
Y659 |
X |
|
|
| Akt1 |
|
|
|
|
| |
T308 |
|
X |
X |
| S473 | X | X |
Time courses have been measured for many of the serine, threonine and tyrosine (S/T/Y) residues considered in the model presented here. Here, we focus on three studies that applied distinct experimental techniques to measure time courses of phosphorylation for specific S/T/Y sites. In the study of Wolf-Yadlin et al. [45], the technique of selected reaction monitoring and quantitative mass spectrometry was applied. In the study of VanMeter et al. [46], the technique of reverse phase protein array was applied. In the study of Ciaccio et al. [47], the technique of microwestern array was applied. An ‘X’ entry in this table signifies that a time course of phosphorylation for the indicated residue was measured in the indicated study.