Table 1.
Sequences of adapters and primers
| Name | Sequence (5' to 3') | Tm (°C) | References |
|---|---|---|---|
| universal adapter-F |
CTCGTAGACTGCGTACCA |
57.30 |
This study |
| universal adapter-R |
TACTCAGGACTCATCGTC |
55.02 |
This study |
| common adapter |
CCTTCCTTCCTTCCCCCC |
61.86 |
Bai et al., 2009 |
| phi085-F |
AGCAGAACGGCAAGGGCTACT |
61.92 |
MaizeGDB |
| phi085-R |
TTTGGCACACCACGACGA |
57.30 |
MaizeGDB |
| U-phi085-F |
CTCGTAGACTGCGTACCAAGCAGAACGGCAAGGGCTACT |
72.61 |
This study |
| U-phi085-R |
TACTCAGGACTCATCGTCTTTGGCACACCACGACGA |
70.05 |
This study |
| C-phi085-F |
CCTTCCTTCCTTCCCCCCAGCAGAACGGCAAGGGCTACT |
74.71 |
This study |
| C-phi085-R |
CCTTCCTTCCTTCCCCCCTTTGGCACACCACGACGA |
73.47 |
This study |
| phi041-F |
TTGGCTCCCAGCGCCGCAAA |
63.95 |
MaizeGDB |
| phi041-R |
GATCCAGAGCGATTTGACGGCA |
61.94 |
MaizeGDB |
| U-phi041-F |
CTCGTAGACTGCGTACCATTGGCTCCCAGCGCCGCAAA |
73.96 |
This study |
| U-phi041-R |
TACTCAGGACTCATCGTCGATCCAGAGCGATTTGACGGCA |
71.33 |
This study |
| C-phi041-F |
CCTTCCTTCCTTCCCCCCTTGGCTCCCAGCGCCGCAAA |
76.12 |
This study |
| C-phi041-R |
CCTTCCTTCCTTCCCCCCGATCCAGAGCGATTTGACGGCA |
74.40 |
This study |
| phi123-F |
GGAGACGAGGTGCTACTTCTTCAA |
61.97 |
MaizeGDB |
| phi123-R |
TGTGGCTGAGGCTAGGAATCTC |
61.94 |
MaizeGDB |
| U-phi123-F |
CTCGTAGACTGCGTACCAGGAGACGAGGTGCTACTTCTTCAA |
71.87 |
This study |
| U-phi123-R |
TACTCAGGACTCATCGTCTGTGGCTGAGGCTAGGAATCTC |
71.33 |
This study |
| C-phi123-F |
CCTTCCTTCCTTCCCCCCGGAGACGAGGTGCTACTTCTTCAA |
73.82 |
This study |
| C-phi123-R |
CCTTCCTTCCTTCCCCCCTGTGGCTGAGGCTAGGAATCTC |
74.40 |
This study |
| umc1478-F |
GAAGCTTCTCCTCTCGCGTCTC |
63.80 |
MaizeGDB |
| umc1478-R |
CAGTCCCAGACCCTAGCTCAGTC |
65.52 |
MaizeGDB |
| U-umc1478-F |
CTCGTAGACTGCGTACCAGAAGCTTCTCCTCTCGCGTCTC |
73.38 |
This study |
| U-umc1478-R |
TACTCAGGACTCATCGTCCAGTCCCAGACCCTAGCTCAGTC |
73.10 |
This study |
| C-umc1478-F |
CCTTCCTTCCTTCCCCCCGAAGCTTCTCCTCTCGCGTCTC |
75.43 |
This study |
| C-umc1478-R |
CCTTCCTTCCTTCCCCCCCAGTCCCAGACCCTAGCTCAGTC |
76.10 |
This study |
| umc1268-F |
ACGAACAACCTAGCACAGTCCTAAA |
60.34 |
MaizeGDB |
| umc1268-R |
CAAGGCGGTTACCAAGTTTACATC |
60.26 |
MaizeGDB |
| U-umc1268-F |
CTCGTAGACTGCGTACCAACGAACAACCTAGCACAGTCCTAAA |
70.70 |
This study |
| U-umc1268-R |
TACTCAGGACTCATCGTCCAAGGCGGTTACCAAGTTTACATC |
69.92 |
This study |
| C-umc1268-F |
CCTTCCTTCCTTCCCCCCACGAACAACCTAGCACAGTCCTAAA |
71.93 |
This study |
| C-umc1268-R |
CCTTCCTTCCTTCCCCCCCAAGGCGGTTACCAAGTTTACATC |
72.85 |
This study |
| phi120-F |
TGATGTCCCAGCTCTGAACTGAC |
61.92 |
MaizeGDB |
| phi120-R |
GACTCTCACGGCGAGGTATGA |
61.95 |
MaizeGDB |
| U-phi120-F |
CTCGTAGACTGCGTACCATGATGTCCCAGCTCTGAACTGAC |
72.10 |
This study |
| U-phi120-R | TACTCAGGACTCATCGTCGACTCTCACGGCGAGGTATGA | 71.56 | This study |
Tms are taken from the primer synthesis report card.